In the objective to identify the less infested cotton varieties by insects, infestation levels in absence of insecticide treatment of 12 varieties in Gobé in centre of Bénin and of 26 varieties in Angaradebou in the north of the country have been evaluated in research station in a complete randomized block design with 5 repetitions in Gobé and 6 repetitions in Angaradebou. The infestation levels of insects (Helicoverpa armigera, Diparopsis watersi, Earias spp, Spodoptera littoralis) which damage cotton reproductive organs were significantly low on GIZA 77, PNS 47 I UI, T1219-153 and SAHR varieties. The infestations of Aphis gossypii, one of the principal insects which damage cotton leaves, were significantly low on MNH 93, LAHG 063, PNS 121 II V-S and R1 R2 Y1 P1 L02 varieties. Agronomic characteristics of the varieties such as number of bolls per plant, quantity of seed cotton per boll, quantity of seed cotton per plant, quantity of seed cotton per hectare and infestation levels of cotton varieties in comparison in Gobé and in Angaradebou showed that the PNS III H H PNS 70 variety was the less infested variety which produced seed cotton per hectare significantly higher than those observed in STAM 18 A.