Acute intoxication and chronic ethanol is recognized by specific symptoms and needs to be confirmed by blood or plasma ethanol dosage. Although, the aim of our paper is to develop and validate a method in the order to determinate the amount of blood ethanol, using gas chromatography with flame ionization detection (FID), after a Headspace sampling. We used butanol as an internal standard, our method is specific and linear at the range amount from 0,1g/L to 5 g/L. the coefficient of correlation was 0.9998. Using the quality control from standard solution we determined the coefficient of variation and recovery percent, compared to the theoretical amount (25%; 50% and 75% of the maximum amount). The coefficient of variation was within 4.046%; 9.682% and 3.553 % at concentration of 0.3; 2.5 and 4 g/L, respectively. The limit of detection was 0.063 g/L. In the same conditions, limit of quantification was 0.085 g/L. The storage's and transport's samples were at +4
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