The fish availability and marketing system at three markets in Barisal were studied for a period of twelve months from August 2012 to July 2013. Data were collected through questionnaire interviews and focus group discussions. A total number of 64 fish species were recorded in the three markets during the study period. Among them highest amounts were ilish (39%) and lowest amounts were Thai koi (2%). About 85% of the fishes were brought from different rivers and ponds of the Barisal region and only 15% from Mymensingh, Jessor, Satkhira and from India (major carps). The marketing channel from fishermen/fish farmer to consumers passes through a number of intermediaries such as local paikers/aratdar/mahajan (local agent), wholesalers and retailers. The average gross profit of fish retailers in Port Road Bazar, Natun Bazar and Lakhutia Bazar were estimated as BDT 950/day, BDT 750/day and BDT 400/day (1 US$=78 BDT), respectively. Lack of storage facilities, poor supply of ice, exploitation by middlemen, lack of money and infrastructure were the common constraints of the fish marketing.