PhD, associated Professor, Department of Mycology and Parasitology and Membership of Infectious Disease and Tropical Medicine Reseach Center, Zahedan University of Medical Sciences, Zahedan, Iran
Trichomonas vaginalis is a protozoan pathogen of the human urogenital tract. This study was designed to provide a data base on investigation of the overall prevalence and clinical findings and compare of diagnosis methods for detection of Trichomoniasis. In this descriptive - cross sectional study a total of 400 vaginal samples were examined with wet smear and Pap smear and diamond culture medium for Trichomoniasis in Chabahar in 2014. The confirmed clinical findings by gynecologist recorded in patient information forms. The data analysis was done using the SPSS software version 18.0 and frequency tables generated. The chi-square and T student tests was used to determine significant relationship between categorical variables at a significant P value of less than or equal to 0.05. In this study number of 39 Trichomoniasis (9.75%) with diamond culture media and 27 cases (6.75%) with Pap smear and 25 cases (6.25%) with wet mounts direct microscopy slides, were reported positive respectively. However, there was no statistically significant difference between the clinical symptoms seen in studied patients with infection (P>0.05), but there was a statistically significant difference between patient reported sings with infection (P<0.05). Considering high prevalence of Trichomonas vaginalis and a substantial proportion of infections are asymptomatic and excessive treatment before the exact laboratory diagnosis makes necessary reliable testing methods. It is recommended in addition to wet smear, culture methods should be performed routinely in medical laboratory.
Oral candidiasis is a common opportunistic infection in diabetic patients. Candida albicans is colonized on different oral surfaces such as tongue, palate, dental caries and plaques. Different factors like age, sex, denture wears and smoking play role in amount and severity of colonization of this microorganism. Our goal was to determine the relationship between the existences of Candida Albicans in oral diabetic patient. This cross - sectional study was performed on 120 diabetic patients. After completing the questionnaire and collect saliva samples and smears from the mouth were cultured directly on sabouraud agar medium containing 0.005% chloramphenicol and for identified use of specific tests. Data were statistically analyzed using Chi-Square, Mann-Whitney, t-test and correlation tests. Overall, in this study from 120 patients, 61 cases (50.8%) had a positive culture for Candida. In this study, Based on observations Candida infection rates were relatively higher in older patients and in women. Infections among participants who used dentures (0.001P <) and have smoking (P <0.038) had significant relationship. The infection in people with type II diabetes is more than subjects with Type I diabetes. C. Albicans was the most common isolated Candida species in both groups. This study showed a high prevalence of Candidiasis and Candida colonization in oral samples of diabetic patients. Our goal was to determine the relationship between the existences of Candida Albicans in oral diabetic patient.