Trichomonas vaginalis is a protozoan pathogen of the human urogenital tract. This study was designed to provide a data base on investigation of the overall prevalence and clinical findings and compare of diagnosis methods for detection of Trichomoniasis. In this descriptive - cross sectional study a total of 400 vaginal samples were examined with wet smear and Pap smear and diamond culture medium for Trichomoniasis in Chabahar in 2014. The confirmed clinical findings by gynecologist recorded in patient information forms. The data analysis was done using the SPSS software version 18.0 and frequency tables generated. The chi-square and T student tests was used to determine significant relationship between categorical variables at a significant P value of less than or equal to 0.05. In this study number of 39 Trichomoniasis (9.75%) with diamond culture media and 27 cases (6.75%) with Pap smear and 25 cases (6.25%) with wet mounts direct microscopy slides, were reported positive respectively. However, there was no statistically significant difference between the clinical symptoms seen in studied patients with infection (P>0.05), but there was a statistically significant difference between patient reported sings with infection (P<0.05). Considering high prevalence of Trichomonas vaginalis and a substantial proportion of infections are asymptomatic and excessive treatment before the exact laboratory diagnosis makes necessary reliable testing methods. It is recommended in addition to wet smear, culture methods should be performed routinely in medical laboratory.