Cleome gynandra L., known as kenebdo in Burkina Faso is an important traditional leafy vegetable for nutritional supplement meant to populations in Burkina Faso where many people, especially children, are suffering from chronic malnutrition. Indeed, due to its high nutritional value, it is a good food supplement in providing nutritional and medicinal needs. This study aims at identifying varieties of interest according to climatic zones of Burkina Faso for varietal improvement of species through multi-local evaluations of a collection of C. gynandra. 36 accessions were basically collected in 12 provinces of Burkina Faso and used as plant material. Experiments were conducted in three sites located in the three climatic zones of Burkina Faso. They were conducted during rainy season using a Fischer block design with three replications. Measurements and observations were made on 16 variables, such as 4 qualitative and 12 quantitative variables. The study revealed great agromorphological variability within the accessions with significant different performances according to the three climatic zones. It also showed significant interaction between accessions and experimental sites for most of the variables. In general, the best agronomic performances were observed in Bobo Dioulasso followed by Ouagadougou while the low performances were recorded in Dori. For each climatic zone, a set of 10 accessions were identified as genotypes of interest that could be used for varietal improvement of C. gynandra in Burkina Faso.