[ Effet inhibiteur d’extraits aqueux de pulpes de caroube, de citron et d’orange sur la cristallurie de patients lithiasiques ]
Volume 27, Issue 2, September 2019, Pages 678–685
Younes AASSEM1, Mohamed BOUHA2, Rachid Bouhdadi3, Mustapha El Bir4, Ahmed GAMOUH5, and Mohamed Mbarki6
1 Laboratoire de Spectro-Chimie Appliquée et Environnement (LSCAE), Département de Chimie et Environnement, Faculté des Sciences et Techniques, Université Sultane Moulay Slimane, Beni Mellal, Morocco
2 Laboratoire des Procèdes Chimiques et Matériaux Appliquée (LPCMA), Département de Chimie et Environnement, Faculté des Sciences et Techniques, Université Sultane Moulay Slimane, Beni Mellal, Morocco
3 Department of chemistry and environment, Sultan Moulay Slimane University, Faculty of Science and Technology, Transdisciplinary Team of Analytical Science for Sustainable Development, PB 523, Beni Mellal, Morocco
4 University of Sultan Moulay Slimane, Faculty of Science and Technology, P.B 523, Beni Mellal, Morocco
5 Laboratoire de Spectro-Chimie Appliquée et Environnement (LSCAE), Département de Chimie et Environnement, Faculté des Sciences et Techniques, Université Sultane Moulay Slimane, Beni Mellal, Morocco
6 Department of chemistry and environment, Sultan Moulay Slimane University, Faculty of Science and Technology, Transdisciplinary Team of Analytical Science for Sustainable Development, PB 523, Beni Mellal, Morocco
Original language: French
Copyright © 2019 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Urolithiasis is defined as the result of abnormal development of the normal constituents of urine within the urinary tract. For a long time, it was called stone sickness, from the Greek "lithos" which means stone. Calcium-calcium lithiasis formed from calcium (Ca) and oxalate (Ox) are by far the most common. The present work is devoted to the study of the inhibitory effect of crystalluria, which may be present in aqueous extracts of fruit byproducts such as carob, lemon and orange pulp. The objective is that the valorization of its agrifood by-products may be related to the antilithiasic effect of their aqueous extracts. Urine samples from human patients were collected in the Regional Hospital Center of the Beni Mellal-Khénifra area in Morocco. The identification of crystalluria and the enumeration of the identified crystals was carried out by polarizing light optical microscope (PLOM). Solutions of aqueous extracts of the pulps of the three fruits were prepared at different concentrations to evaluate the count, on the PLOM, of calcium oxalate crystals formed. The pulps of the three by-products (carob, lemon and orange) have an inhibitory effect for the formation of crystals, especially in the case of lemon. According to the obtained results, it is found that at the 0.25 g / l concentration of the aqueous extract, for each of the three types of carob, lemon and orange pulp, around 50% of the number of the calcium oxalate disappears. In addition, it is found that for the aqueous extract of carob pulp at the three concentrations 0.25, 0.125 and 0.0625 g / l, comparing the results on the aqueous solution of calcium oxalate and the urine of the patient lithiasic, we note that the inhibitory effect is not clear. However, the two extracts of lemon and orange pulps have an inhibitory effect on crystalluria for the three concentrations of the extracts.
Author Keywords: antilithiasic effect, calcium oxalate, crystalluria, by-products, carob, lemon, orange, light microscopy.
Volume 27, Issue 2, September 2019, Pages 678–685
Younes AASSEM1, Mohamed BOUHA2, Rachid Bouhdadi3, Mustapha El Bir4, Ahmed GAMOUH5, and Mohamed Mbarki6
1 Laboratoire de Spectro-Chimie Appliquée et Environnement (LSCAE), Département de Chimie et Environnement, Faculté des Sciences et Techniques, Université Sultane Moulay Slimane, Beni Mellal, Morocco
2 Laboratoire des Procèdes Chimiques et Matériaux Appliquée (LPCMA), Département de Chimie et Environnement, Faculté des Sciences et Techniques, Université Sultane Moulay Slimane, Beni Mellal, Morocco
3 Department of chemistry and environment, Sultan Moulay Slimane University, Faculty of Science and Technology, Transdisciplinary Team of Analytical Science for Sustainable Development, PB 523, Beni Mellal, Morocco
4 University of Sultan Moulay Slimane, Faculty of Science and Technology, P.B 523, Beni Mellal, Morocco
5 Laboratoire de Spectro-Chimie Appliquée et Environnement (LSCAE), Département de Chimie et Environnement, Faculté des Sciences et Techniques, Université Sultane Moulay Slimane, Beni Mellal, Morocco
6 Department of chemistry and environment, Sultan Moulay Slimane University, Faculty of Science and Technology, Transdisciplinary Team of Analytical Science for Sustainable Development, PB 523, Beni Mellal, Morocco
Original language: French
Copyright © 2019 ISSR Journals. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Urolithiasis is defined as the result of abnormal development of the normal constituents of urine within the urinary tract. For a long time, it was called stone sickness, from the Greek "lithos" which means stone. Calcium-calcium lithiasis formed from calcium (Ca) and oxalate (Ox) are by far the most common. The present work is devoted to the study of the inhibitory effect of crystalluria, which may be present in aqueous extracts of fruit byproducts such as carob, lemon and orange pulp. The objective is that the valorization of its agrifood by-products may be related to the antilithiasic effect of their aqueous extracts. Urine samples from human patients were collected in the Regional Hospital Center of the Beni Mellal-Khénifra area in Morocco. The identification of crystalluria and the enumeration of the identified crystals was carried out by polarizing light optical microscope (PLOM). Solutions of aqueous extracts of the pulps of the three fruits were prepared at different concentrations to evaluate the count, on the PLOM, of calcium oxalate crystals formed. The pulps of the three by-products (carob, lemon and orange) have an inhibitory effect for the formation of crystals, especially in the case of lemon. According to the obtained results, it is found that at the 0.25 g / l concentration of the aqueous extract, for each of the three types of carob, lemon and orange pulp, around 50% of the number of the calcium oxalate disappears. In addition, it is found that for the aqueous extract of carob pulp at the three concentrations 0.25, 0.125 and 0.0625 g / l, comparing the results on the aqueous solution of calcium oxalate and the urine of the patient lithiasic, we note that the inhibitory effect is not clear. However, the two extracts of lemon and orange pulps have an inhibitory effect on crystalluria for the three concentrations of the extracts.
Author Keywords: antilithiasic effect, calcium oxalate, crystalluria, by-products, carob, lemon, orange, light microscopy.
Abstract: (french)
La lithiase urinaire est définie comme le résultat d’un développement anormal des constituants normaux de l’urine à l’intérieur du tractus urinaire. Pendant longtemps, elle a été appelée maladie de la pierre, du grec « lithos » qui signifie la pierre. Les lithiases oxalo-calciques formées à partir du calcium (Ca) et d’oxalate (Ox) sont de loin les plus fréquentes. Le présent travail est consacré à l’étude l’effet inhibiteur, de la cristallurie, que peuvent présenter des extraits aqueux de sous-produits de fruits tels que les pulpes de caroube et de citron et d’orange. L’objectif consiste au fait que la valorisation de ses sous-produits agroalimentaires puisse être liée à l’effet antilithiasique de leurs extraits aqueux. Les échantillons d’urines de patients humains ont été collectés dans le Centre Hospitalier Régional de la région de Béni Mellal-Khénifra au Maroc. L’identification de la cristallurie et le dénombrement des cristaux identifiés a été réalisée par microscope optique à lumière polarisée (MOLP). Des solutions d’extraits aqueux des pulpes des trois fruits ont été préparées à différentes concentrations permettant d’évaluer le dénombrement, par la MOLP, des cristaux d’oxalate de calcium formés. Les pulpes des trois sous-produits (caroube, citron et l’orage) ont un effet inhibiteur pour la formation des cristaux, notamment dans le cas du citron. D’après les résultats obtenus on constate qu’à la concentration de 0,25g /l de l’extrait aqueux, pour chacun des trois types pulpes : de caroube, de citron et d’orange, environs 50% du nombre des cristaux de l’oxalate de calcium disparait. En plus, on constate que pour l’extrait aqueux des pulpes de caroube aux trois concentrations 0,25, 0,125 et 0,0625 g/l, en comparant les résultats sur la solution aqueuse d’oxalate de calcium et l’urine du patient lithiasique, on remarque que l’effet inhibiteur est non net. Cependant, les deux extraits des pulpes de citron et orange présentent un effet inhibiteur de la cristallurie, pour les trois concentrations des extraits.
Author Keywords: effet antilithiasique, oxalate de calcium, cristallurie, sous-produits, caroube, citron, orange, microscopie optique.
How to Cite this Article
Younes AASSEM, Mohamed BOUHA, Rachid Bouhdadi, Mustapha El Bir, Ahmed GAMOUH, and Mohamed Mbarki, “Inhibitory effect of aqueous extracts for carob, lemon and orange pulp on the crystalluria of lithiasic patients,” International Journal of Innovation and Applied Studies, vol. 27, no. 2, pp. 678–685, September 2019.
