Urolithiasis is defined as the result of abnormal development of the normal constituents of urine within the urinary tract. For a long time, it was called stone sickness, from the Greek "lithos" which means stone. Calcium-calcium lithiasis formed from calcium (Ca) and oxalate (Ox) are by far the most common. The present work is devoted to the study of the inhibitory effect of crystalluria, which may be present in aqueous extracts of fruit byproducts such as carob, lemon and orange pulp. The objective is that the valorization of its agrifood by-products may be related to the antilithiasic effect of their aqueous extracts. Urine samples from human patients were collected in the Regional Hospital Center of the Beni Mellal-Khénifra area in Morocco. The identification of crystalluria and the enumeration of the identified crystals was carried out by polarizing light optical microscope (PLOM). Solutions of aqueous extracts of the pulps of the three fruits were prepared at different concentrations to evaluate the count, on the PLOM, of calcium oxalate crystals formed. The pulps of the three by-products (carob, lemon and orange) have an inhibitory effect for the formation of crystals, especially in the case of lemon. According to the obtained results, it is found that at the 0.25 g / l concentration of the aqueous extract, for each of the three types of carob, lemon and orange pulp, around 50% of the number of the calcium oxalate disappears. In addition, it is found that for the aqueous extract of carob pulp at the three concentrations 0.25, 0.125 and 0.0625 g / l, comparing the results on the aqueous solution of calcium oxalate and the urine of the patient lithiasic, we note that the inhibitory effect is not clear. However, the two extracts of lemon and orange pulps have an inhibitory effect on crystalluria for the three concentrations of the extracts.

The majority of the analyzed calculi from patients are composed of calcium oxalate (CaOx) monohydrate whewellite (Wh) and CaOx dihydrate wedellite (Wd). The urinary calculi were identified by chemical and morphological analysis based on106 urine samples from human voluntary. The Crystalluria made by an optical polarized light microscopy. The oxaluria and urinary calcium were determined by conventional volumetric assays. The aim of this paper was to develop a simple system to predict and classify the type of crystalluria using Artificial Neural Networks (ANNs) algorithm.

In the context of sustainable development and promoting natural resources, some forgotten fruits such as the Zizyphus Lotus can be developed as local products. In addition, the frequency of Urolithiasis multiplied increasingly rapidly in the world. The Urolithiasis involves the formation of crystalline aggregates called "urinary stones" developed in the urinary tract, usually in the kidneys or ureters, but may also affect the bladder or urethra. The objective of this study is to study in vitro the crystallization of calcium oxalate as a lithogenic species and to investigate the inhibitory effect of aqueous jujube extracts on the crystallization of calcium oxalate in order to exploit it. Six jujube fruit samples were taken from six geographical zones from Beni Mellal-Khenifra region. These fruit - seed samples were separated from their seeds. The pulps were subjected to a grinding mortar so as to have fine powder. The samples have been subsequently, submitted a cold maceration during 48 hours. The study of the crystallization of calcium oxalate is carried out by the optical microscope with polarized light (MLP). Some aqueous extracts have an anti-lithiasic effect on the aqueous solution of the lithogenous species studied.