Objective: Serological diagnosis of 48 suspected cases of Whooping Cough, received Medical Bacteriology Department at the National Institute of Hygiene in Rabat from four Moroccan provinces. The technique used is the ELISA. Results: From 48 suspected cases of Whooping Cough, 28 cases (58%) were positives, 10 cases (21%) were negatives and 10 cases (21%) were doubtful. Conclusion: Despite the effort of the Ministry of Health for immunization against pertussis immunization coverage which exceeds 90% of children, we see sporadic cases affecting some provinces that may be responsible for death.

Pertussis or whooping cough is a highly contagious infection of the respiratory tract caused by the bacterium albordtella pertussis. It’s a disease that could affects human at all his stages of life and the infection may be repeated several times, but gravity is reflected in babies given the complications that can lead to death. Over the last decades, the disease have increased although vaccines. And because of the decreased immunity against this disease among adults and adolescents, making them vulnerable, they become a source of infection for children who have not yet received the vaccine or have not completed the initial vaccination.

Production of extended-spectrum beta-lactamases (ESBL) considered one of the most important resistance mechanisms that impair antimicrobial treatment of infections caused by Enterobacteriaceae. Four phenotypic methods were compared to detect ESBL production; the tests based on the synergy between a third-generation cephalosporin and clavulanate. These tests are: the double-disk synergy test (DDST) 25 to 30mm, DDST (30mm), DDST (20mm) and the double-disk (Spanish- test). In our study, we worked on 81 strains of ESBL enterobacteriaceae. Synergy test 25 to 30 mm, could detect 90.12% of ESBLs strains. So if we had known the best distance which we will clearly detected the "champagne cork" appearance, we practiced synergy test at 30mm. This test highlighted ESBL production in only 17 (20.99%) strains. distance reduction between C3G disks and clavulanate disk to 20 mm led to detect 73 (90.12%). Double disk test (Spanish test) detected 81 (100%), this test confirms the presence of ESBLS. By comparison with other tests, this test had the highest rate of ESBLS. In most cases; standard disk diffusion tests are effective, and still recommended for ESBL detection in routine laboratories. Nevertheless, it is worth combining standard disk diffusion test with other approaches, such as modified disk tests or E tests.

A total of 37 clinical isolates of enterobacteriaceaes (16 Escherichia coli, 10 Klebsiella pn., 7 Enterobacter Cloacae, 2 Morganella Morganii, 1 Proteus Mirabilis and 1 Citrobacter Freundi ) were recovered during 2014 from four Moroccan regions to study their resistance profile. Among these 37 strains, 19 tell ESBL Enterobacteriaceae. Over this period, the bacterial species most often ESBL producing was Escherichia coli (9 strains), followed by Klebsiella pneumoniae (7 strains), Enterobacter cloacae (2 strains), and Morganella Morganii (1 strains). The ESBL resistance was screened using disc diffusion method, while the resistance genes were detected by poly

Serology plays an important role in the diagnosis of leptospirosis. Few laboratories have the resources and expertise to perform the microscopic agglutination test and leptospirosis remains a neglected disease because of limited access to diagnosis, due to reliance on antiquated standard methods and the cost of commercially available alternatives. There is a need for rapid and simple serological tests which facilitate the early diagnosis of leptospirosis, while antibiotic therapy may be most effective. In Morocco this disease is little known. Studies about it are very rare. In this study 11 serums which referred to the National Institute of Health in Rabat, Morocco during 1-1-2014 to 30-6-2015 were evaluated by IgM ELISA and Slide agglutination test (SAT). 7 serums were positives by Elisa and 10 were positives by (SAT). 9 of cases were from Sidi kecem region. All patients were male. The rate of age for all patients was 29.5 years.

The objective of this work is the study of the physico-chemical and microbiological quality of a derivative of Moroccan dates: Dkess (date paste). This is the basis for Food Saharan regions. Despite the sensitivity of Dkess to alteration and that poses serious problems to human health found that few studies conducted on this product. The study was performed on 300 samples, physico-chemical characteristics (4 criteria), microbiological and hygienic (10 criteria) were assessed against the standards. A Characterization of microbial flora and a study of the antibiotic susceptibility of some isolated bacteria were performed. The results showed that the quality of pulp is much more developed than that of traditional pulp and semi industrialized and this on all the criteria examined whether physicochemical or microbiological (42% of the samples of traditional pulp and 33% of dough semi industrialized does not conform to international standards). In addition, the poor preservation of places of production causes an alteration of traditional pasta and pasta semi industrialized dates and their susceptibility to contamination by microorganisms, and the damage is even more important as storage conditions and storage are not adequate. The control of manufacturing processes and preparation as well as the entire food chain of these products must be improved to ensure the health and safety of consumers.

The objective of this work is the study of the quality of a derivative of Moroccan dates: Tahlaoute. It is a vital and essential product oasis whose population exceeds two million. However, this product has been a little or no studies. The study was performed on 220 samples (110 samples of traditional Tahlaoute and 110 samples of industrialized Tahlaoute), the physicochemical characteristics (4 criteria), the microbiological and hygienic (10 criteria) were assessed against the standards. The results showed that the quality of industrialized Tahlaoute is much larger than traditional Tahlaoute and this on all criteria studied whether physico- chemical or microbiological (60% of samples of traditional Tahlaoute are not consistent with international standards). In addition, storage conditions are generally unfavorable, resulting in an alteration of traditional Tahlaoute and its susceptibility to contamination by microorganisms. The control of manufacturing processes and preparation as well as the entire food chain of these products must be improved to ensure the health and safety of consumers.